Informatics Publishing Limited

Pallavi S. Jamadagni ¹, Sharad D. Pawar ¹, Shrirang Jamadagni ¹, Shridhar Chougule ¹, Manish Wanjari ¹, Manish Gautam ², Sudesh Gaidhani ³, Arun Gurav ¹, N. Shrikanth ³

Affiliations
1 Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, IN
2 Assam Down Town University, Panikhaiti, Guwahati - 781026, Assam, IN
3 Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, IN

Abstract

The in-vitrocytotoxicity was studied in a pancreatic cell line (RIN5F) using streptozotocin-induced cellular damage. The Cytotoxic dose of aqueous and hydro-alcoholic extracts of Mimosa pudica root powder before and after streptozotocin treatment was studied and protein expression was explored. Both the extracts were subjected to metabolite analysis by LCMS. Mimosa pudica root powder extracts prevented Streptozotocin-induced cellular damage. Proteomics studies indicated both aqueous and hydro-alcoholic extracts of Mimosa pudica regulate proteins related to insulin secretion, glucose uptake, and insulin resistance with marked changes in proteins involved in insulin secretion.  The study first time reports cytotoxicity of Mimosa pudica in RIN5F cells and explores protein expression after Mimosa pudica treatment. It was also observed that the abundance of Mimosine is more in Hydro-alcoholic extracts as compared to aqueous extracts.

Keywords

LC-MS, Mimosa pudica, Proteomics, RIN5F.

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Dipsundar Sahu ¹, Manajit Bora ¹, A. K. Dixit ¹, Shrirang Jamadagni ², Manosi Das ¹, Narasimhaji Cheemalapati , Barnail Sinha , Qadir Alam ¹, Sharad Pawar ¹, P. V.Vara Prasad ¹

Affiliations
1 Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., IN
2 Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Kothrud, Pune, Maharashtra 411038., IN

Abstract

Skin burn is a common health problem that needs a fast and accurate treatment; else, it can have a worse prognosis. The available treatment options pose limitations due to their adverse effects; thus there is an urgent need to find newer treatment options for burn wound healing that can provide a safer and faster treatment option. Ayurveda gives a plethora of medicinal plants and their formulations which have been used in the management of burn wound. The Ayurvedic ointment formulated in this experiment utilizes essential ayurvedic ingredients which are known for their burn wound healing potential. The current work includes the formulation of the Ayurvedic ointment, its standardization, toxicity and efficacy study. The Ayurvedic ointment was formulated by fusion method, and its physicochemical characterization ensures the purity of the compounds used for the formulation. The ointment formulated is well set under the safety parameters for the presence of heavy metals and also free from microbial and fungal contamination. It has obtained optimum spreadability and viscosity, making it desirable for application on wounds. In the acute dermal toxicity study, it has shown no adverse effect on the animals as there were no significant changes in the body weight and feed intake and water consumption, thus confirming it as a safe formulation. It has shown significant rate of wound healing and period of re-epithelization in the Wistar rat model. It can be a potential ointment for burn wound treatment, and in future, it can be considered for clinical studies to validate its safety and efficacy in humans.

Keywords

Burn wound, Ayurveda, Ointment, Fusion method, Re-epithelization.

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Sudesh N. Gaidhani ¹, Srikanth Ala ¹, Viswanth Reddy ¹, Y. R. Sanjaya Kumar ¹, Shrirang Jamadagni ¹, Thamizh Selvam ¹, K. K. Ajeesh Kumar ¹

Affiliations
1 Pharmacology, National Ayurveda Research Institute for Panchakarma, Cheruthuruthy, Thrissur - 679531, Kerala, IN

Abstract

Background: VasantKusumakar Ras (VK Ras) is a traditional Ayurvedic preparation used in the treatment of Type-2 diabetes mellitus. Despite its clinical anti-diabetic claims, no pre-clinical attempts were made to rule out its efficacy as an antidiabetic agent. Objectives: The present study was carried out to find the anti-diabetic effect of VK Ras against a High-Fat Diet (HFD), and low-dose streptozotocin (STZ) induced type 2 diabetes and to explore the mode of action of VK Ras. Materials and Methods: Different doses of VK Ras were administered to diabetic rats for 35 days. The biochemical markers analysis, intestinal glucose uptake, and liver glycogen content were estimated at the end of the study and also vital organs were weighed and subjected to histopathological evaluation. Results: VK Ras treatment reduced blood glucose in a dose-dependent manner. The insulin, HbA1C, HOMA-IR, and lipid profiles were improved in VK Ras-treated animals as compared to diabetic control animals. The relative organ weights were changed in diabetic rats, and treatment with VK Ras corrected the organ weights. Intestinal glucose uptake and liver glycogen content were decreased with treatment. Further, the histopathological analysis of the pancreas and other vital organs had shown that dose-dependent restoration of organ function with VK Ras treatment. Conclusions: VK Ras treatment reduces insulin resistance as well as corrects the lipid, hepatic and renal abnormalities that arise from diabetes, these effects may be mediated by interfering with glucose transport from the gut and insulin release from the β pancreatic cells.

Keywords

HbA1C, HFD, HOMA-IR, Insulin, STZ, VK Ras

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